This practical manual on protein research is designed to be a progression of experimental protocols that an inexperienced investigator may follow when embarking on a biochemical or biotechnology project. The reader is guided through the plethora of proven and productive methods for both soluble proteins and membrane or particulate-associated proteins. Special attention is given to providing optimalchoices from a range of methods available in a modern lab for a particular application. Over 180 protocols are included with ...
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This practical manual on protein research is designed to be a progression of experimental protocols that an inexperienced investigator may follow when embarking on a biochemical or biotechnology project. The reader is guided through the plethora of proven and productive methods for both soluble proteins and membrane or particulate-associated proteins. Special attention is given to providing optimalchoices from a range of methods available in a modern lab for a particular application. Over 180 protocols are included with materials lists, step-by-step instructions and comments on practical issues such as time considerations and safety issues. Each chapter includes troubleshooting tips, icons and references. Selected chapters and protocols may serve as lab courses in contmporary protein research techniques.
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