Notes on Histological Methods; Including a Brief Consideration of the Methods of Pathological and Vegetable Histology and the Application of the Microscope to Jurisprudence
Notes on Histological Methods; Including a Brief Consideration of the Methods of Pathological and Vegetable Histology and the Application of the Microscope to Jurisprudence
This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1886 Excerpt: ...and placed immediately in 40 to 50 times its volume of the hardening agent. As longitudinal rugae of the stomach are usually present it is better to make transections. Imbed, section, etc., as in (38-46. Stain with haematoxylin, or alum carmine 2 to 3 minutes and fluorescine 15 to 30 seconds. Or stain 5 to 10 minutes ...
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This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1886 Excerpt: ...and placed immediately in 40 to 50 times its volume of the hardening agent. As longitudinal rugae of the stomach are usually present it is better to make transections. Imbed, section, etc., as in (38-46. Stain with haematoxylin, or alum carmine 2 to 3 minutes and fluorescine 15 to 30 seconds. Or stain 5 to 10 minutes with picrocarmine. References: Quain, II, 593; Sat. 388; Str. 370; F1. 430; St. 178; K. 196; (Ar. m. a. (1885) 174). 54) Setflion of the ileum.--The sections should be near the caecum where the villi are comparatively few. Either transections or longisections may be made. If longisections are to be made the ileum should be cut lengthwise and pinned out flat during the hardening and imbedding processes. The dorsal or attached aspect of the terminal part of the ileum usually contains so many Peyer's follicles that the relations of the villi and crypts of Lieberkiihn are obscured, hence sections of the ventral or free aspect are to be preferred, or if transections are made they should include the ventral part. Some of the sections should be thick, .04 mm., others should be very thin. Stain with haematoxylin or alum carmine, 2 to 3 minutes. References: Quain, II, 599; S2. 146; Heitz. 597; Str. 380; K. 201; F. & L. 132. ( 55) Section of liver.--Harden, imbed, etc., as directed in 37-46. Stain with haematoxylin 3 to 5 minutes, or with alum carmine the same time, and then 15 to 30 seconds with fluorescine. References: Quain, II, 626; Heitz. 675; B., 159; Str. 407. 56) Setflion of ovary.--The greatest care should be taken not to touch the surface of the ovary in the various processes, as there is danger of removing the surface epithelium. Imbed and place in the microtome so that transections shall be made. Some of the sections should be as thin as p..
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