This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1904 edition. Excerpt: ... or upon various methods of embalming the nervous system in situ. The results and the extent of some lesions are well studied in this way. Many data concerning causes are, however, lost. III. MINUTE LESIONS OF THE NERVOUS SYSTEM. METHODS OF EXAMINATION. The pia mater, vessels, and connective tissues are ...
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This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1904 edition. Excerpt: ... or upon various methods of embalming the nervous system in situ. The results and the extent of some lesions are well studied in this way. Many data concerning causes are, however, lost. III. MINUTE LESIONS OF THE NERVOUS SYSTEM. METHODS OF EXAMINATION. The pia mater, vessels, and connective tissues are examined by the methods employed for other organs. Fixation in Zenker.s fluid, paraffin imbedding, and staining with methylene blue and eosin form a good method at the outset. The staining with methylene blue and eosin is supplemented by the aniline blue connective tissue stain (Mallory). Arteriosclerosis is so large a factor in many lesions that the elastic tissue stain of Weigert must frequently be used. For the study of the neuroglia and ependyma, fixation in Zenker.s fluid permits the use of the phosphotungstic acid hematein method (Mallory). Weigert's stain for neuroglia may be employed. These methods will not succeed unless the tissue has been fixed within a few hours post mortem. Fibrin stains and the iron hematoxylin method may be employed. The study of the cell bodies of neuroglia cells is best carried on in sections stained with methylene blue and eosin. The study of the nerve cells may be adequately made with sections stained with methylene blue and eosin after fixation in Zenker.s fluid. Excellent work is done with the classical method of Nissl (alcohol fixation, staining with methylene blue soap solution), and, for work on nerve cells which depends for interpretation on comparative studies in the literature, Nissl.s method should be used. The manifold methods used by histologists in making clear the structure of the nerve cell are unfortunately of little avail in pathological work, as their results are too precarious or require...
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