This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein ...
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This detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Third Edition serves as an ideal guide for scientists aiming to produce and purify functional recombinant membrane proteins for structural studies.
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Add this copy of Heterologous Expression of Membrane Proteins: Methods to cart. $103.32, new condition, Sold by Ingram Customer Returns Center rated 5.0 out of 5 stars, ships from NV, USA, published 2018 by Humana.
Add this copy of Heterologous Expression of Membrane Proteins: Methods to cart. $103.32, new condition, Sold by Ingram Customer Returns Center rated 5.0 out of 5 stars, ships from NV, USA, published 2016 by Humana.
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New. Print on demand Sewn binding. Cloth over boards. 322 p. Contains: Unspecified, Illustrations, black & white, Illustrations, color, Tables, black & white, Tables, color. Methods in Molecular Biology, 1432.
Add this copy of Heterologous Expression of Membrane Proteins to cart. $106.63, new condition, Sold by discount_scientific_books rated 4.0 out of 5 stars, ships from Sterling Heights, MI, UNITED STATES, published 2009 by Humana.
Add this copy of Heterologous Expression of Membrane Proteins: Methods to cart. $112.72, new condition, Sold by Ingram Customer Returns Center rated 5.0 out of 5 stars, ships from NV, USA, published 2016 by Humana.
Add this copy of Heterologous Expression of Membrane Proteins: Methods to cart. $159.69, new condition, Sold by Ingram Customer Returns Center rated 5.0 out of 5 stars, ships from NV, USA, published 2023 by Springer-Verlag New York Inc..
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New. Print on demand Contains: Illustrations, black & white, Illustrations, color. Methods in Molecular Biology . XVII, 467 p. 94 illus., 78 illus. in color. Intended for professional and scholarly audience.
Add this copy of Heterologous Expression of Membrane Proteins: Methods to cart. $159.69, new condition, Sold by Ingram Customer Returns Center rated 5.0 out of 5 stars, ships from NV, USA, published 2009 by Humana.
Add this copy of Heterologous Expression of Membrane Proteins: Methods to cart. $191.10, new condition, Sold by Booksplease rated 3.0 out of 5 stars, ships from Southport, MERSEYSIDE, UNITED KINGDOM, published 2023 by Springer-Verlag New York Inc..
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New. Contains: Illustrations, black & white, Illustrations, color. Methods in Molecular Biology . XVII, 467 p. 94 illus., 78 illus. in color. Intended for professional and scholarly audience.
Add this copy of Heterologous Expression of Membrane Proteins: Methods to cart. $193.06, good condition, Sold by Bonita rated 4.0 out of 5 stars, ships from Newport Coast, CA, UNITED STATES, published 2023 by Humana.
Add this copy of Heterologous Expression of Membrane Proteins: Methods to cart. $234.84, new condition, Sold by Ingram Customer Returns Center rated 5.0 out of 5 stars, ships from NV, USA, published 2022 by Springer-Verlag New York Inc..
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Seller's Description:
New. Print on demand Contains: Illustrations, black & white, Illustrations, color. Methods in Molecular Biology . XVII, 467 p. 94 illus., 78 illus. in color. Intended for professional and scholarly audience.
Add this copy of Heterologous Expression of Membrane Proteins: Methods to cart. $298.93, new condition, Sold by Booksplease rated 3.0 out of 5 stars, ships from Southport, MERSEYSIDE, UNITED KINGDOM, published 2022 by Springer-Verlag New York Inc..
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Seller's Description:
New. Contains: Illustrations, black & white, Illustrations, color. Methods in Molecular Biology . XVII, 467 p. 94 illus., 78 illus. in color. Intended for professional and scholarly audience.
