The book begins by describing in detail the rationale behind designing gene targeting vectors, including both basic replacement vectors for creating null mutations and more sophisticated two-step approaches for creating subtle mutations. It describes the latest techniques for culturing and manipulating ES cells and a number of approaches for identifying rare targeted cell clones. Techniques for culturing and assaying for mouse and human hematopoietic progenitors in vitro and in vivo are then covered, followed by the ...
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The book begins by describing in detail the rationale behind designing gene targeting vectors, including both basic replacement vectors for creating null mutations and more sophisticated two-step approaches for creating subtle mutations. It describes the latest techniques for culturing and manipulating ES cells and a number of approaches for identifying rare targeted cell clones. Techniques for culturing and assaying for mouse and human hematopoietic progenitors in vitro and in vivo are then covered, followed by the design of, and use of, retrovirus vectors for introducing and expressing genes in bone marrow cells. Two approaches for making ES cell chimeras are described. One chapter covers the more standard approach of ES cell injection into blastocysts as well as morula injection. Another chapter describes techniques for making aggregation chimeras and in particular a recent approach using tetraploid host embryos to produce completely ES cell-derived embryos and mice. The critical steps for obtaining germline chimeras are discussed. The book is concluded by a discussion of the many uses of enhancer, gene and promoter trap vectors, the advantages of each, and describes in detail the techniques for producing and analyzing "trap" vector insertions in ES cells. The protocols are written with sufficient detail for a novice in the area to use them as a manual to learn the various techniques required for producing mutant mice, beginning with the embryonic stem cells and ending up with chimeric mice for breeding.
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Add this copy of Gene Targeting: a Practical Approach to cart. $3.28, like new condition, Sold by ThriftBooks-Atlanta rated 5.0 out of 5 stars, ships from Austell, GA, UNITED STATES, published 2000 by Oxford University Press, USA.
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Very Good. Very Good condition. 2nd edition. A copy that may have a few cosmetic defects. May also contain light spine creasing or a few markings such as an owner's name, short gifter's inscription or light stamp. Bundled media such as CDs, DVDs, floppy disks or access codes may not be included.
Add this copy of Gene Targeting: a Practical Approach (the ^Apractical to cart. $6.99, good condition, Sold by HPB-Red rated 5.0 out of 5 stars, ships from Dallas, TX, UNITED STATES, published 1993 by Oxford University Press.
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Add this copy of Gene Targeting: a Practical Approach to cart. $19.27, good condition, Sold by Anybook rated 5.0 out of 5 stars, ships from Lincoln, UNITED KINGDOM, published 2000 by Oxford University Press, Usa.
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This is an ex-library book and may have the usual library/used-book markings inside. This book has soft covers. In good all round condition. Please note the Image in this listing is a stock photo and may not match the covers of the actual item, 650grams, ISBN: 9780199637928.
Add this copy of Gene Targeting: a Practical Approach to cart. $25.59, fair condition, Sold by Anybook rated 5.0 out of 5 stars, ships from Lincoln, UNITED KINGDOM, published 2001 by Oxford University Press.
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This is an ex-library book and may have the usual library/used-book markings inside. This book has soft covers. Clean from markings. In fair condition, suitable as a study copy. Please note the Image in this listing is a stock photo and may not match the covers of the actual item, 600grams, ISBN: 9780199637928.
Add this copy of Gene Targeting: a Practical Approach to cart. $31.42, good condition, Sold by Bonita rated 4.0 out of 5 stars, ships from Newport Coast, CA, UNITED STATES, published 2000 by Oxford University Press.
Add this copy of Gene Targeting: a Practical Approach (the ^Apractical to cart. $34.31, good condition, Sold by Bonita rated 4.0 out of 5 stars, ships from Newport Coast, CA, UNITED STATES, published 1993 by Oxford University Press.
