2.1 Introduction ........................................................... 32 2.2 Technical Procedures ................................................... 32 Chemical Synthesis ..................................................... 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 38 . . . . . . . . . . . . . Chemical Synthesis ..................................................... 38 Binding to anti-CCK antisera and CCK-B receptors .......................... 40 Binding to ...
Read More
2.1 Introduction ........................................................... 32 2.2 Technical Procedures ................................................... 32 Chemical Synthesis ..................................................... 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 38 . . . . . . . . . . . . . Chemical Synthesis ..................................................... 38 Binding to anti-CCK antisera and CCK-B receptors .......................... 40 Binding to streptavidin agarose and photoelution ........................... 40 Affinity chromatography ................................................. 41 2.4 Troubleshooting....................................................... 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands ......................... 45 Summary ............................................................. 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures ................................................... 48 Preparation of a Biotinylated Ligand ...................................... 48 Preparation of Membrane Fraction ........................................ 50 Solubilization of the PACAP Receptor ..................................... 51 Partial Purification of the PACAP Receptor ................................. 52 Affinity Purification of the PACAP Receptor ................................ 54 Final Purification of the PACAP Receptor .................................. 56 Receptor-Binding Assay ................................................. 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting ....................................................... 61 Impurity in PACAP27-Cys-NH............................................ 61 2 Low specific activity of the PACAP receptor despite a single band ............. 62 Acknowledgments ..................................................... 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ......... " 65 Summary ............................................................. 65 4.1 Introduction................................................ . . . . . . . .. . 66 . 4.2 Technical Procedures ................................................. " 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ............. 67 Synthesis of photo reactive biotinylated peptide hormones ................... 67 Site-specific incorporation of biotin and photo labels in separate steps ........ 69 Photoaffinity labeling ................................................... 72 4.3 Results and Discussion .................................................. 76 Synthesis of photoactivatable insulins with permanent biotin labels ............ 76 Applications: Insulin .................................................... 78 VI Examples for other applications .......................................... 78 4.4 Troubleshooting....................................................... 79 References . . . . . . . . . . . . . . . . . . . . .
Read Less
Book Details
Seller
Sort
Seller Rating
Price: Low to High
Price: High to Low
Condition
Condition: Reverse
Pub Date
Pub Date: Reverse
Sellers Near Me
Trade paperback,
New
2012, Birkhauser
ISBN-13:
9783034873512
See Item Details ▾
Alibris
BEST
NV, USA
$51.65
$99.00
Add to Cart
Add this copy of A Laboratory Guide to Biotin-Labeling in Biomolecule to cart. $51.65, new condition, Sold by Ingram Customer Returns Center rated 5.0 out of 5 stars, ships from NV, USA, published 2012 by Birkhauser.
Edition:
2012, Birkhauser
Trade paperback,
New
Available Copies: 10+
Details:
ISBN:
3034873514
ISBN-13:
9783034873512
Pages:
240
Edition:
1996 edition
Publisher:
Birkhauser
Published:
2012
Language:
English
Alibris ID:
11914984719
Shipping Options:
Standard Shipping: $4.99
Trackable Expedited: $9.99
Two Day Air: $29.99
Choose your shipping method in Checkout. Costs may vary based on destination.
Seller's Description:
New. Trade paperback (US). Glued binding. 240 p. Contains: Unspecified. Biomethods.
Hide Details ▴
Trade paperback,
New
2012, Birkhauser
ISBN-13:
9783034873512
See Item Details ▾
GreatBookPrices
HIGH
Columbia,
MD,
USA
$58.06
$99.00
Add to Cart
Add this copy of A Laboratory Guide to Biotin-Labeling in Biomolecule to cart. $58.06, new condition, Sold by GreatBookPrices rated 4.0 out of 5 stars, ships from Columbia, MD, UNITED STATES, published 2012 by Birkhauser.
Edition:
2012, Birkhauser
Trade paperback,
New
Available Copies: 3
Details:
ISBN:
3034873514
ISBN-13:
9783034873512
Pages:
240
Edition:
1996 edition
Publisher:
Birkhauser
Published:
2012
Language:
English
Alibris ID:
17988013343
Shipping Options:
Standard Shipping: $4.99
Choose your shipping method in Checkout. Costs may vary based on destination.
Seller's Description:
New. Trade paperback (US). Glued binding. 240 p. Contains: Unspecified. Biomethods. In Stock. 100% Money Back Guarantee. Brand New, Perfect Condition, allow 4-14 business days for standard shipping. To Alaska, Hawaii, U.S. protectorate, P.O. box, and APO/FPO addresses allow 4-28 business days for Standard shipping. No expedited shipping. All orders placed with expedited shipping will be cancelled. Over 3, 000, 000 happy customers.
Hide Details ▴
Trade paperback,
Good
2012, Birkhauser
ISBN-13:
9783034873512
See Item Details ▾
Bonita
HIGH
Newport Coast,
CA,
USA
$80.09
$99.00
Add to Cart
Add this copy of A Laboratory Guide to Biotin-Labeling in Biomolecule to cart. $80.09, good condition, Sold by Bonita rated 4.0 out of 5 stars, ships from Newport Coast, CA, UNITED STATES, published 2012 by Birkhäuser.
Edition:
2012, Birkhauser
Trade paperback,
Good
Details:
ISBN:
3034873514
ISBN-13:
9783034873512
Pages:
240
Edition:
1996 edition
Publisher:
Birkhauser
Published:
2012
Language:
English
Alibris ID:
17906522332
Shipping Options:
Standard Shipping: $4.99
Trackable Expedited: $9.99
Choose your shipping method in Checkout. Costs may vary based on destination.
Seller's Description:
Good. Access codes and supplements are not guaranteed with used items. May be an ex-library book.
Hide Details ▴
2012,
Birkhauser, Basel
ISBN-13: 9783034873512
1996 edition
Trade paperback