Since the polymerase chain reaction (PCR) was first developed in 1985, an enormous number of research reports have documented the versatility of this brilliant technique for in vitro amplification of nucleic acids. Although PCR has had a profound impact in many areas of research, contrary to exp- tation its routine application to the quantitation of nucleic acids has proven problematic in several aspects. The shortcomings are principally caused by the exponential nature of PCR, whereby small variations in amplification ef- ...
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Since the polymerase chain reaction (PCR) was first developed in 1985, an enormous number of research reports have documented the versatility of this brilliant technique for in vitro amplification of nucleic acids. Although PCR has had a profound impact in many areas of research, contrary to exp- tation its routine application to the quantitation of nucleic acids has proven problematic in several aspects. The shortcomings are principally caused by the exponential nature of PCR, whereby small variations in amplification ef- ciency may dramatically affect the yield of amplification product. Even mi- mal temperature deviations that occur between adjacent wells of a thermocycler or day-to-day variations in the efficiency of nucleic acid preparation can lead to significant differences in the extent of amplification between otherwise id- tical samples. However, knowing more about the intrinsic limitations of PCR is the first step towards surmounting the shortcomings associated with this prom- ing methodology. With the introduction of appropriate standards of known amount, which are co-amplified with the sample using the same primers, it is increasingly feasible to address biological or diagnostic questions that are d- ficult or impossible to answer using any other experimental approach.
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Add this copy of Quantitative Pcr Protocols to cart. $4.17, very good condition, Sold by ThriftBooks-Reno rated 5.0 out of 5 stars, ships from Reno, NV, UNITED STATES, published 1999 by Humana Press.
Add this copy of Quantitative Pcr Protocols (Methods in Molecular to cart. $8.26, very good condition, Sold by Goodwill of Silicon Valley rated 5.0 out of 5 stars, ships from San Jose, CA, UNITED STATES, published 1628 by Humana Press.
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Supports Goodwill of Silicon Valley job training programs. The cover and pages are in very good condition! The cover and any other included accessories are also in very good condition showing some minor use. The spine is straight there are no rips tears or creases on the cover or the pages.
Add this copy of Quantitative Pcr Protocols (Methods in Molecular to cart. $50.00, very good condition, Sold by Eastburn Books rated 4.0 out of 5 stars, ships from Albany, OR, UNITED STATES, published 1999 by Humana Press.
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Very Good. Book VG hardcover. 1999. About 10 pages with hard crease/bump to corner. Pages clean/unmarked. Covers bright/clean. LIght scuffs. Binding solid. Diagrams throughout. 304 pp.
